Fig 1: Western blot analysis and relative fold protein expression of GPR81, P-CREB, CREB, and the ratios of P-PKA/PKA and P-CREB/CREB after chronic administration. (A) Western blot analysis of GPR81 and proteins involved in cAMP-PKA pathway. (B) Fold protein expression of GPR81. (C) The ratio of P-PKA/PKA. (D) Fold protein expression of P-CREB. (E) Fold protein expression of CREB. (F) The ratio of P-CREB/CREB. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. * p<0.05.